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  • Probing for Mitochondrial Complex Activity in Human Embryonic Stem Cells Watch

In this video, we will show you how the mitochondrial respiratory chain complexes of human embryonic stem cells can be analyzed using in gel activity assays.

  • From MEFs to Matrigel 3: Passaging hESCs from Matrigel onto Matrigel Watch

This video demonstrates how to maintain the growth of human embryonic stem cells (hESCs) in feeder cell-free conditions and how to continuously passage hESCs in feeder cell-free conditions. Confirmation of hESC pluripotency grown in feeder cell-free conditions by immunofluorescence microscopy is also demonstrated.

  • From MEFs to Matrigel 2: Splitting hESCs from MEFs onto Matrigel Watch

This video demonstrates how to maintain the growth of human embryonic stem cells (hESCs) in feeder cell-free conditions and how to continuously passage hESCs in feeder cell-free conditions. Confirmation of hESC pluripotency grown in feeder cell-free conditions by immunofluorescence microscopy is also demonstrated.

  • From MEFs to Matrigel I: Passaging hESCs in the Presence of MEFs Watch

This video demonstrates how to grow human embryonic stem cells (hESCs) on mouse embryonic fibroblast (MEF) feeder cells.